hdr plasmid (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
hdr plasmid
Hdr Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdr+plasmids/Heme+Oxygenase+1+HDR+Plasmid/pm41934679-54-19-12
Average 94 stars, based on 3 article reviews
Hdr Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdr+plasmids/Heme+Oxygenase+1+HDR+Plasmid/pm41934679-54-19-12
Average 94 stars, based on 3 article reviews
hdr plasmid - by Bioz Stars,
2026-09
94/100 stars
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Transfection:Article Title: Loss of PIK3CA allows in vitro growth but not in vivo progression of KRAS mutant lung adenocarcinoma in a syngeneic orthotopic implantation model Article Snippet: .. KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Self-Amplifying NRF2-EZH2 Epigenetic Loop Converts KRAS Initiated Progenitors to Invasive Pancreatic Cancer Article Snippet: Acinar, ductal and EPCAM + cells were separated as described 28 , 49 , 50 after staining with biotinylated Dolichos Biflorus Agglutinin (DBA) (Vector Labs, B-1035), biotinylated Ulex Europaeus Agglutinin I (UEA I) (Vector Laboratories, B-1065), or biotin anti-CD326 (EpCAM) (eBioscience, #13-5791-80), followed by immunomagnetic biotin positive selection (EasySep, StemCell Technologies, # 18556) according to manufacturer’s instructions. .. Cell lines were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) with the following CRISPR/Cas9 KO and Article Title: A nuclease-driven mechanism of post-replicative ssDNA gap suppression Article Snippet: .. Stable MRNIP KO CRISPR clones were generated via transfection of HeLa cells with Santa Cruz Biotechnology CRISPR-Cas9 gRNA and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD) Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Loss of PIK3CA Allows In Vitro Growth but Not In Vivo Progression of KRAS Mutant Lung Adenocarcinoma in a Syngeneic Orthotopic Implantation Model. Article Snippet: .. Generation of KPS Cells KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD). Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout and siRNA transfection CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Loss of PIK3CA Allows In Vitro Growth but Not In Vivo Progression of KRAS Mutant Lung Adenocarcinoma in a Syngeneic Orthotopic Implantation Model Article Snippet: .. KP cells were similarly transfected with CRISPR/Cas9 knockout and CRISPR:Article Title: Loss of PIK3CA allows in vitro growth but not in vivo progression of KRAS mutant lung adenocarcinoma in a syngeneic orthotopic implantation model Article Snippet: .. KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Self-Amplifying NRF2-EZH2 Epigenetic Loop Converts KRAS Initiated Progenitors to Invasive Pancreatic Cancer Article Snippet: Acinar, ductal and EPCAM + cells were separated as described 28 , 49 , 50 after staining with biotinylated Dolichos Biflorus Agglutinin (DBA) (Vector Labs, B-1035), biotinylated Ulex Europaeus Agglutinin I (UEA I) (Vector Laboratories, B-1065), or biotin anti-CD326 (EpCAM) (eBioscience, #13-5791-80), followed by immunomagnetic biotin positive selection (EasySep, StemCell Technologies, # 18556) according to manufacturer’s instructions. .. Cell lines were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) with the following CRISPR/Cas9 KO and Article Title: A nuclease-driven mechanism of post-replicative ssDNA gap suppression Article Snippet: .. Stable MRNIP KO CRISPR clones were generated via transfection of HeLa cells with Santa Cruz Biotechnology CRISPR-Cas9 gRNA and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD) Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Loss of PIK3CA Allows In Vitro Growth but Not In Vivo Progression of KRAS Mutant Lung Adenocarcinoma in a Syngeneic Orthotopic Implantation Model. Article Snippet: .. Generation of KPS Cells KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD). Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout and siRNA transfection CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Loss of PIK3CA Allows In Vitro Growth but Not In Vivo Progression of KRAS Mutant Lung Adenocarcinoma in a Syngeneic Orthotopic Implantation Model Article Snippet: .. KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Propionyl-CoA carboxylase subunit B regulates anti-tumor T cells in a pancreatic cancer mouse model Article Snippet: .. Pik3ca −/− (αKO) KPC cell lines were generated by transfecting WT KPC cells with Pik3ca CRISPR/Cas9 αKO and Knock-Out:Article Title: Loss of PIK3CA allows in vitro growth but not in vivo progression of KRAS mutant lung adenocarcinoma in a syngeneic orthotopic implantation model Article Snippet: .. KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD) Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Loss of PIK3CA Allows In Vitro Growth but Not In Vivo Progression of KRAS Mutant Lung Adenocarcinoma in a Syngeneic Orthotopic Implantation Model. Article Snippet: .. Generation of KPS Cells KP cells were similarly transfected with CRISPR/Cas9 knockout and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD). Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout and siRNA transfection CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Loss of PIK3CA Allows In Vitro Growth but Not In Vivo Progression of KRAS Mutant Lung Adenocarcinoma in a Syngeneic Orthotopic Implantation Model Article Snippet: .. KP cells were similarly transfected with CRISPR/Cas9 knockout and Plasmid Preparation:Article Title: Self-Amplifying NRF2-EZH2 Epigenetic Loop Converts KRAS Initiated Progenitors to Invasive Pancreatic Cancer Article Snippet: Acinar, ductal and EPCAM + cells were separated as described 28 , 49 , 50 after staining with biotinylated Dolichos Biflorus Agglutinin (DBA) (Vector Labs, B-1035), biotinylated Ulex Europaeus Agglutinin I (UEA I) (Vector Laboratories, B-1065), or biotin anti-CD326 (EpCAM) (eBioscience, #13-5791-80), followed by immunomagnetic biotin positive selection (EasySep, StemCell Technologies, # 18556) according to manufacturer’s instructions. .. Cell lines were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) with the following CRISPR/Cas9 KO and Control:Article Title: Self-Amplifying NRF2-EZH2 Epigenetic Loop Converts KRAS Initiated Progenitors to Invasive Pancreatic Cancer Article Snippet: Acinar, ductal and EPCAM + cells were separated as described 28 , 49 , 50 after staining with biotinylated Dolichos Biflorus Agglutinin (DBA) (Vector Labs, B-1035), biotinylated Ulex Europaeus Agglutinin I (UEA I) (Vector Laboratories, B-1065), or biotin anti-CD326 (EpCAM) (eBioscience, #13-5791-80), followed by immunomagnetic biotin positive selection (EasySep, StemCell Technologies, # 18556) according to manufacturer’s instructions. .. Cell lines were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) with the following CRISPR/Cas9 KO and Clone Assay:Article Title: A nuclease-driven mechanism of post-replicative ssDNA gap suppression Article Snippet: .. Stable MRNIP KO CRISPR clones were generated via transfection of HeLa cells with Santa Cruz Biotechnology CRISPR-Cas9 gRNA and Generated:Article Title: A nuclease-driven mechanism of post-replicative ssDNA gap suppression Article Snippet: .. Stable MRNIP KO CRISPR clones were generated via transfection of HeLa cells with Santa Cruz Biotechnology CRISPR-Cas9 gRNA and Article Title: Propionyl-CoA carboxylase subunit B regulates anti-tumor T cells in a pancreatic cancer mouse model Article Snippet: .. Pik3ca −/− (αKO) KPC cell lines were generated by transfecting WT KPC cells with Pik3ca CRISPR/Cas9 αKO and Selection:Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD) Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD). Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout and siRNA transfection CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Knockdown:Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD) Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD). Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout and siRNA transfection CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Western Blot:Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD) Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and Article Title: Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD). Article Snippet: Total RNA was extracted using TRIzol (Ambion, 1596026, Austin, TX, USA) and purified with the Direct-zol RNA Miniprep Kit (Zymo Research, R2052, Irvine, CA, USA). cDNA was synthesized from 3 μg of RNA using TOPscript RT drymix (Enzynomics, RT200, Daejeon, Korea). qRT-PCR was performed using SYBR Green Master Mix (Enzynomics, RT500U), and gene expression was normalized to β-actin or RPLP0. .. CRBN knockout and siRNA transfection CRBN knockout was performed in HEK293T, U87, H1299, and Caki-1 cells using a CRISPR/Cas9 system (Santa Cruz, sc-412142). sgCRBN/Cas9 and |
